Interaction between bradykinin and urodilatin - a possible mechanism of clinical relevance
نویسندگان
چکیده
Background Bradykinin (BK) plays a significant role in pathophysiology of different diseases from angioedema to brain stroke and heart attack by inducing vasodilatation and increasing capillary permeability. We investigate the potential effects of natriuretic peptides on BK signaling by measuring membrane potential (Vm) of HEK293 cells using the whole cell patch clamp technique. HEK293cells are an excellent model since they display the natriuretic peptide receptor type A as well as both BK receptors (BR1 and BR2).
منابع مشابه
Preparation of a Photoactivable Ligand for Bradykinin Receptors
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In this study, the interaction of natriuretic peptides (NP) and bradykinin (BK) signaling pathways was identified by measuring membrane potential (V(m)) and intracellular Ca(2+) using the patch-clamp technique and flow cytometry in HEK-293 cells. BK and NP receptor mRNA was identified using RT-PCR. BK (100 nM) depolarized cells activating bradykinin receptor type 2 (B(2)R) and Ca(2+)-dependent ...
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